{"id":10408,"date":"2019-12-18T10:50:05","date_gmt":"2019-12-18T15:50:05","guid":{"rendered":"https:\/\/ibol.org\/?p=10408"},"modified":"2019-12-18T11:11:01","modified_gmt":"2019-12-18T16:11:01","slug":"watered-down-biodiversity","status":"publish","type":"post","link":"https:\/\/ibol.org\/site\/watered-down-biodiversity\/","title":{"rendered":"Watered down biodiversity: sample type is critical in environmental DNA studies for biomonitoring"},"content":{"rendered":"\n\n[et_pb_section fb_built=”1″ _builder_version=”3.22.7″ custom_padding=”0px||0px|||” hover_enabled=”0″][et_pb_row column_structure=”1_2,1_2″ use_custom_gutter=”on” custom_padding_last_edited=”on|desktop” _builder_version=”4.0.9″ background_color=”#141418″ use_background_color_gradient=”on” background_color_gradient_start=”rgba(20,20,24,0.71)” background_color_gradient_end=”rgba(0,0,0,0)” background_color_gradient_direction=”90deg” background_color_gradient_end_position=”72%” background_color_gradient_overlays_image=”on” background_image=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2019\/12\/DSCN1264-scaled.jpg” custom_padding=”|||100px” custom_padding_tablet=”|30px||30px” custom_padding_phone=”” hover_enabled=”0″ height=”444px”][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”3.29.3″ _dynamic_attributes=”content” text_font=”Oswald|||on|||||” text_text_color=”#ffffff” text_font_size=”29px” text_line_height=”1.3em” header_font=”||||||||”]@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF90aXRsZSIsInNldHRpbmdzIjp7ImJlZm9yZSI6IiIsImFmdGVyIjoiIn19@[\/et_pb_text][et_pb_text _builder_version=”3.27.4″ _dynamic_attributes=”content”]@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9jYXRlZ29yaWVzIiwic2V0dGluZ3MiOnsiYmVmb3JlIjoiIiwiYWZ0ZXIiOiIiLCJsaW5rX3RvX3Rlcm1fcGFnZSI6Im9uIiwic2VwYXJhdG9yIjoiIHwgIiwiY2F0ZWdvcnlfdHlwZSI6ImNhdGVnb3J5In19@[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22.3″ custom_padding=”3px|0px|0px|0px|false|false”][et_pb_row _builder_version=”3.25″ custom_padding=”31px|0px|0|0px|false|false”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.0.9″ _dynamic_attributes=”content” text_font=”||on||||||” text_text_color=”#646564″ custom_padding=”20px||” hover_enabled=”0″]@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9kYXRlIiwic2V0dGluZ3MiOnsiYmVmb3JlIjoiIiwiYWZ0ZXIiOiIiLCJkYXRlX2Zvcm1hdCI6ImRlZmF1bHQiLCJjdXN0b21fZGF0ZV9mb3JtYXQiOiIifX0=@[\/et_pb_text][et_pb_text _builder_version=”4.0.9″ text_font=”||||||||” custom_padding=”0px||18px|||” text_font_size_tablet=”” text_font_size_phone=”16px” text_font_size_last_edited=”on|phone”]

<\/strong><\/em><\/p>\n

DNA-based biomonitoring relies on species-specific segments of organisms DNA for their taxonomic identification and is rapidly advancing for monitoring invertebrate communities across a variety of ecosystems. The analytical approaches taken vary from single-species detection to bulk environmental sample analysis, depending largely on the focus of data generation. However, for freshwater systems, there is often a lack of consideration as to the optimal sample type for maximising detection of macroinvertebrates.<\/p>\n

Ecology, life stage and habitat preference (i.e. benthic or water column) of macroinvertebrates ultimately influences the rate of DNA detection depending on the sampling approach taken. DNA-based biomonitoring data collected for freshwater macroinvertebrates often focuses on detection of groups Ephemeroptera (mayflies), Plecoptera (stoneflies), Trichoptera (caddisflies) and Odonata (dragonflies & damselflies), to infer the health status of freshwater systems.<\/p>\n

In their larval stage, these macroinvertebrates \u2013 commonly referred to as EPTO \u2013 occupy the benthos in rivers, lakes, ponds, and wetlands. Considering this, water samples have been proposed as a surrogate source of macroinvertebrate DNA, despite lack of understanding as to whether water provides sufficient taxonomic recovery of macroinvertebrates, particularly of EPTO groups.<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”3.25″ custom_padding=”0px|0px|0px|0px|false|false”][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.0.9″ text_font=”||||||||” custom_padding=”0px||41px|||” text_font_size_tablet=”” text_font_size_phone=”16px” text_font_size_last_edited=”on|phone”]

To address this, a recent collaboration between the Hajibabaei Lab<\/a> (Centre for Biodiversity Genomics, University of Guelph) and scientists at Environment and Climate Change Canada resulted in a paper in PLOS One investigating the recovery of macroinvertebrates, in particular EPTO, in shallow open-water wetlands by comparing matched water and bulk-tissue DNA samples.<\/p>\n

Overall, they found that very few taxa were shared between bulk-benthos and water samples, with a much greater richness of macroinvertebrate taxa recovered from benthos. EPTO groups in particular were associated strongly with bulk-benthos samples, with limited EPTO families detected in all matched water samples.<\/p>\n

\u201cThis pristine, wetland study system is excellent for comparing the relative detection of these taxa without the influence of water flow,\u201d said lead author Prof. Mehrdad Hajibabaei. The study illustrates how sample choice is a critical factor for a comprehensive assessment of total macroinvertebrate biodiversity. \u201cThis research is vitally important for informing large-scale projects such as STREAM<\/a>, where a high volume of benthic macroinvertebrate data is now being generated using a standardised DNA-based methodology.\u201d<\/p>[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2019\/12\/IMG_6375-scaled-e1576608471340.jpg” _builder_version=”4.0.9″][\/et_pb_image][et_pb_text _builder_version=”4.0.9″]

Prof. Mehrdad Hajibabaei. Photo courtesy of the Centre for Biodiversity Genomics.<\/em><\/span><\/p>\n

<\/em><\/span><\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.0.9″ custom_padding=”0px|||||”][et_pb_column type=”1_2″ _builder_version=”4.0.9″][et_pb_image src=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2019\/12\/Chloe-Robinson-1-scaled.jpg” _builder_version=”4.0.9″][\/et_pb_image][et_pb_text _builder_version=”4.0.9″]

Dr. Chloe Robinson. Photo courtesy of the Centre for Biodiversity Genomics.<\/em><\/span><\/p>[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.0.9″][et_pb_text _builder_version=”4.0.9″]

\u201cSpecies detectability is an important consideration when designing biomonitoring programs,\u201d said Dr. Donald Baird, co-author and Research Scientist with Environment and Climate Change Canada\u2019s Water Science and Technology Directorate. \u201cOur study shows clearly that to access macroinvertebrates DNA water samples are no substitute for bulk organism collection, as the majority of critical indicator taxa are simply not detected when we know they are present.\u201d<\/p>\n

Eliminating false negatives and positives is crucial for creating high quality baseline data for determining the health status of Canadian watersheds. \u201cThere is a need for consistency of biomonitoring data when assessing total biodiversity, and bulk-benthos samples provide sufficient taxonomic coverage that is both cost-effective and efficient,\u201d said Dr. Chloe Robinson, co-author and project manager for STREAM.<\/p>\n

This study emphasises the critical nature of choosing representative sampling methods to maximise DNA capture from target organisms whilst avoiding diluting the diversity, to enable informed decisions regarding freshwater health.<\/p>\n

To read more on STREAM, visit:\u00a0<\/span>www.STREAM-DNA.com<\/a><\/p>\n

Follow @STREAM_CBG on Twitter<\/a> and Instagram<\/a><\/p>\n

Follow @hajibabaeilab<\/a> on Twitter<\/p>\n

To read more publications by the Hajibabaei Lab, visit: http:\/\/hajibabaei.ibarcode.org\/publications\/<\/a><\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22.3″ custom_padding=”0px|||||”][et_pb_row _builder_version=”3.25″ background_color=”#a8d367″ custom_padding=”67px|0px|0|0px|false|false” border_style_top=”double”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”||||||||” text_orientation=”center” text_font_size_tablet=”” text_font_size_phone=”16px” text_font_size_last_edited=”on|desktop”]

Media Contacts: <\/strong><\/p>\n

International Barcode of Life Consortium<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ custom_padding_last_edited=”on|phone” _builder_version=”3.25″ background_color=”#a8d367″ custom_padding=”0px||” custom_padding_tablet=”8px|||0px” custom_padding_phone=”0px|||0px”][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”Abel||||||||” link_font=”||||||||” link_text_color=”#646564″ header_font=”||||||||” text_orientation=”center” module_alignment=”right” custom_padding_tablet=”” custom_padding_phone=”20px||” custom_padding_last_edited=”on|phone”]

Hannah James<\/strong>
Manager –\u00a0Media and Strategic Communications
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Mehrdad Hajibabaei, PhD<\/b>
Associate Professor
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A recent collaboration between the University of Guelph and scientists at Environment and Climate Change Canada resulted in a paper in PLOS One comparing water and bulk-tissue DNA samples.<\/p>\n","protected":false},"author":9,"featured_media":10445,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[13],"tags":[],"class_list":["post-10408","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/posts\/10408","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/comments?post=10408"}],"version-history":[{"count":13,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/posts\/10408\/revisions"}],"predecessor-version":[{"id":10454,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/posts\/10408\/revisions\/10454"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/media\/10445"}],"wp:attachment":[{"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/media?parent=10408"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/categories?post=10408"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/tags?post=10408"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}