{"id":17,"date":"2017-03-14T17:49:23","date_gmt":"2017-03-14T17:49:23","guid":{"rendered":"http:\/\/ibol.org\/site\/?page_id=17"},"modified":"2020-12-02T09:36:33","modified_gmt":"2020-12-02T14:36:33","slug":"dna-barcoding","status":"publish","type":"page","link":"https:\/\/ibol.org\/site\/about\/dna-barcoding\/","title":{"rendered":"DNA barcoding"},"content":{"rendered":"

[et_pb_section fb_built=”1″ fullwidth=”on” _builder_version=”4.7.4″ background_color=”#ffffff” use_background_color_gradient=”on” background_color_gradient_start=”#003254″ background_color_gradient_end=”#a8d367″ background_color_gradient_direction=”145deg” background_color_gradient_start_position=”34%” background_size=”contain” background_position=”top_right” module_alignment=”right” custom_margin=”|||” custom_margin_last_edited=”off|desktop” custom_padding=”|||” bottom_divider_color=”#f2f2f2″ bottom_divider_height=”72px” bottom_divider_flip=”vertical” hover_enabled=”0″ max_height=”300px” sticky_enabled=”0″][et_pb_fullwidth_header title=”DNA Barcoding” _builder_version=”3.18.9″ title_font=”|||on|||||” title_text_color=”#ffffff” title_font_size=”40px” content_font=”|||on|||||” content_text_color=”#ffffff” background_color=”rgba(168,211,103,0)” background_layout=”light” module_alignment=”left” custom_padding=”|||” title_font_size_tablet=”” title_font_size_phone=”30px” title_font_size_last_edited=”on|phone” content_font_size_tablet=”” content_font_size_phone=”16px” content_font_size_last_edited=”on|desktop” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”]A tool for specimen identification and species discovery
\n[\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”3.22″ background_color=”#ffffff” background_size=”contain” background_position=”top_center” custom_padding=”1px|0px|0|0px|false|false” top_divider_color=”#ffffff” bottom_divider_style=”ramp2″ bottom_divider_color=”#ffffff”][et_pb_row admin_label=”row” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_padding=”67px|0px|21px|0px|false|false”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text admin_label=”DNA Barcodes” _builder_version=”3.27.4″ header_font=”||||||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” header_font_size_tablet=”25px” header_font_size_phone=”20px” header_font_size_last_edited=”on|tablet”]<\/p>\n

The Process<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row make_equal=”on” disabled_on=”on|off|off” _builder_version=”3.25″ background_image=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2018\/11\/aboutDNAbarcoding.png” background_size=”contain” custom_padding=”100px||” custom_css_main_element=”margin:auto;” column_structure=”1_4,1_4,1_4,1_4″][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_divider show_divider=”off” _builder_version=”3.19.4″ height=”100px”][\/et_pb_divider][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_divider show_divider=”off” admin_label=”Divider” _builder_version=”3.19.4″][\/et_pb_divider][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_divider show_divider=”off” admin_label=”Divider” _builder_version=”3.19.4″][\/et_pb_divider][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_divider show_divider=”off” admin_label=”Divider” _builder_version=”3.19.4″ max_width_tablet=”50%” max_width_phone=”” max_width_last_edited=”on|desktop” module_alignment=”right” height=”100px” custom_margin=”-124px|||-17px” link_option_url=”http:\/\/v4.boldsystems.org\/index.php\/Public_BarcodeCluster?clusteruri=BOLD:AAB2508″][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row make_equal=”on” disabled_on=”off|on|on” _builder_version=”3.25″ background_image=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2019\/01\/Barcodeprocess-ver.png” background_size=”contain” custom_padding=”241px|0px|0|0px|false|false” custom_css_main_element=”margin:auto;” column_structure=”1_4,1_4,1_4,1_4″][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_divider show_divider=”off” _builder_version=”3.19.4″][\/et_pb_divider][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_divider show_divider=”off” _builder_version=”3.19.4″][\/et_pb_divider][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_divider show_divider=”off” _builder_version=”3.19.4″][\/et_pb_divider][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_divider show_divider=”off” admin_label=”Divider” _builder_version=”3.19.4″ max_width_tablet=”50%” max_width_phone=”28%” max_width_last_edited=”on|desktop” module_alignment=”center” height=”100px” custom_margin=”8px|||” custom_padding=”20px|||13px” link_option_url=”http:\/\/v4.boldsystems.org\/index.php\/Public_BarcodeCluster?clusteruri=BOLD:AAB2508″][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ custom_padding=”22px|0px|67px|0px|false|false”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”||||||||” header_font=”||||||||” text_orientation=”center” text_font_size_tablet=”” text_font_size_phone=”16px” text_font_size_last_edited=”on|tablet”]<\/p>\n

Step 1:\u00a0<\/strong>Isolate DNA from the sample
Step 2:<\/strong>\u00a0Amplify the target DNA barcode region using PCR
Step 3:<\/strong> Sequence the PCR products
Step 4:<\/strong>\u00a0Compare the resulting sequences against reference databases to find the matching species<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ prev_background_color=”#ffffff” admin_label=”section” _builder_version=”3.22″ background_color=”#f2f2f2″ background_size=”contain” background_position=”top_center” custom_padding=”24px|0px|0|0px|false|false” top_divider_style=”slant2″ bottom_divider_flip=”horizontal”][et_pb_row module_id=”DNAbarcode” _builder_version=”3.25″ custom_padding=”67px|0px|14px|0px|false|false”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text admin_label=”DNA Barcodes” _builder_version=”3.27.4″ header_font=”||||||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” header_font_size_tablet=”25px” header_font_size_phone=”20px” header_font_size_last_edited=”on|phone”]<\/p>\n

The DNA Barcode<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ custom_padding=”|||” column_structure=”1_3,1_3,1_3″][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2018\/10\/Plantbarcode.png” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ box_shadow_style=”preset1″] 
\n[\/et_pb_image][et_pb_text _builder_version=”3.27.4″ text_font=”||||||||” text_orientation=”center” text_font_size_tablet=”” text_font_size_phone=”16px” text_font_size_last_edited=”on|desktop”]<\/p>\n

Plant barcoding studies use one or a few plastid regions (e.g. rbcL and matK, and the non-coding spacer trnH-psbA) and the internal transcribed spacer (ITS) region of nuclear ribosomal DNA.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2018\/10\/AnimalBarcode.png” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ box_shadow_style=”preset1″] 
\n[\/et_pb_image][et_pb_text _builder_version=”3.27.4″ text_font=”Noto Serif||||||||” text_orientation=”center” text_font_size_tablet=”” text_font_size_phone=”16px” text_font_size_last_edited=”on|desktop”]<\/p>\n

Animal barcoding studies use a region in the mitochondrial cytochrome c<\/em> oxidase 1 gene (\u201cCO1\u201d).<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2018\/10\/Fungibarcode.png” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ box_shadow_style=”preset1″] 
\n[\/et_pb_image][et_pb_text _builder_version=”3.27.4″ text_font=”||||||||” header_font=”||||||||” header_text_align=”center” text_orientation=”center” text_font_size_tablet=”” text_font_size_phone=”16px” text_font_size_last_edited=”on|desktop”]Fungal barcoding studies use the internal transcribed spacer (ITS) region in the nuclear ribosomal cistron. This region shows reasonable discriminatory power at the species level in many groups.
\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ prev_background_color=”#f2f2f2″ admin_label=”section” _builder_version=”3.22″ background_color=”#ffffff” background_size=”contain” background_position=”top_center” custom_padding=”|||” top_divider_style=”slant2″ top_divider_flip=”horizontal”][et_pb_row module_id=”DNAlibrary” _builder_version=”3.25″ column_structure=”1_2,1_2″][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”||||||||” header_font=”||||||||” text_font_size_tablet=”” text_font_size_phone=”16px” text_font_size_last_edited=”on|desktop” header_font_size_tablet=”25px” header_font_size_phone=”20px” header_font_size_last_edited=”on|phone”]<\/p>\n

The Library<\/h1>\n

Barcode sequences are placed in the\u00a0Barcode of Life Data Systems (BOLD)\u00a0database \u2013 an online workbench that includes a reference library of DNA barcodes that can be used to assign identities to sequences of unknown origin.<\/p>\n

BOLD is a searchable repository for barcode records, storing specimen data and images as well as sequences and trace files. It provides an identification engine based on the current barcode library and monitors the number of barcode sequence records and species coverage.[\/et_pb_text][et_pb_button button_url=”http:\/\/v4.boldsystems.org\/” url_new_window=”on” button_text=”Become a BOLD user” button_alignment=”center” _builder_version=”3.16″ custom_button=”on” button_font=”||||||||” button_icon=”%%32%%” button_on_hover=”off” button_text_color_hover=”#ffffff” button_bg_color_hover=”#a8d367″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”on” button_text_color__hover=”#ffffff” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”on” button_bg_color__hover=”#a8d367″ button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”] 
\n[\/et_pb_button][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2018\/10\/BOLD.png” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ border_width_all=”1px” border_color_all=”#646564″] 
\n[\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ fullwidth=”on” prev_background_color=”#ffffff” _builder_version=”3.22″ background_color=”#f2f2f2″ custom_padding=”45px|0px|0|0px|false|false” top_divider_style=”slant2″ bottom_divider_color=”#f2f2f2″][et_pb_fullwidth_header title=”The Applications” button_one_text=”learn more” button_one_url=”https:\/\/ibol.org\/impact\/barcoding-applications\/” module_id=”DNAapplication” _builder_version=”3.19.7″ title_font=”||||||||” content_font=”||||||||” background_color=”rgba(168,211,103,0)” custom_button_one=”on” button_one_text_color=”#a8d367″ button_one_font=”||||||||” button_one_icon=”%%32%%” button_one_icon_color=”#a8d367″ button_one_on_hover=”off” background_layout=”light” title_font_size_tablet=”25px” title_font_size_phone=”20px” title_font_size_last_edited=”on|phone” content_font_size_tablet=”” content_font_size_phone=”16px” content_font_size_last_edited=”on|phone” button_one_text_color_hover=”#ffffff” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_one_text_color__hover_enabled=”on” button_one_text_color__hover=”#ffffff” button_two_text_color__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”]<\/p>\n

iBOL\u00a0researchers have applied DNA barcoding to real-world problems with broad impacts on all areas in which society interacts with biodiversity \u2013 pest and disease control, food production and safety, resource management, conservation, research, education, and recreation.<\/p>\n

[\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” padding_top_1=”40px” padding_top_2=”14px” _builder_version=”3.19.4″ background_color=”#3c3d41″ custom_margin=”0px||” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop” custom_padding=”0|0px|0|0px|false|false” global_module=”3909″ saved_tabs=”all”][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” padding_top__hover=”40px” parallax__hover=”off” parallax_method__hover=”on” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ibol.org\/site\/wp-content\/uploads\/2018\/10\/iBOL-logo-vertical-small-copy.png” url=”https:\/\/ibol.org\/” align=”center” admin_label=”iBOL logo” _builder_version=”3.23″ max_width_tablet=”100%” max_width_phone=”30%” max_width_last_edited=”on|tablet” module_alignment=”center” custom_margin=”|||” custom_margin_tablet=”|||0px” custom_margin_phone=”||-67px|-2px” custom_margin_last_edited=”on|tablet” custom_padding=”||50px|” custom_padding_tablet=”||0px|0px” custom_padding_phone=”|0px||” custom_padding_last_edited=”on|phone” always_center_on_mobile=”off”][\/et_pb_image][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”3.25″ custom_padding=”|||” padding_top__hover=”14px” parallax__hover=”off” parallax_method__hover=”on” custom_padding__hover=”|||”][et_pb_row_inner _builder_version=”3.25″ max_width_tablet=”100%” max_width_phone=”100%” max_width_last_edited=”on|desktop” module_alignment=”center” custom_margin_tablet=”-64px|0px||0px” custom_margin_phone=”0px|||0px” custom_margin_last_edited=”on|tablet” custom_padding=”|0px||0px”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”3.25″ custom_padding=”|||” parallax__hover=”off” parallax_method__hover=”on” custom_padding__hover=”|||”][et_pb_text admin_label=”Footer Links” _builder_version=”4.4.7″ text_font=”Montserrat|500||on|||||” text_text_color=”#aaaaaa” text_font_size=”15px” text_line_height=”2.2em” header_font=”Montserrat||||||||” text_orientation=”center” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”10px” text_font_size_last_edited=”on|desktop”]HOME<\/a><\/span>PROGRAMS <\/a><\/span>DNA BARCODING <\/a><\/span>NEWS & EVENTS <\/a><\/span>JOIN<\/a><\/span>CONTACT<\/a><\/span>SITEMAP<\/a>[\/et_pb_text][et_pb_divider color=”#666666″ color_tablet=”” color_phone=”rgba(0,0,0,0)” color_last_edited=”on|desktop” _builder_version=”3.24″ height=”0px” custom_margin_tablet=”” custom_margin_phone=”||-26px” custom_margin_last_edited=”on|desktop” hide_on_mobile=”off”][\/et_pb_divider][et_pb_text admin_label=”Copyright” _builder_version=”4.1″ text_font=”Source Sans Pro||||” text_text_color=”#aaaaaa” text_font_size=”16″ text_line_height=”1.5em” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”center” max_width=”560px” module_alignment=”center” custom_margin=”||30px|” custom_margin_tablet=”||10px|” custom_margin_phone=”” custom_margin_last_edited=”on|tablet” custom_padding=”30px|||” custom_padding_tablet=”” custom_padding_phone=”14px||” custom_padding_last_edited=”on|desktop” text_font_size_tablet=”” text_font_size_phone=”13px” text_font_size_last_edited=”on|desktop” use_border_color=”off” border_color=”#ffffff” border_style=”solid”]<\/p>\n

\u00a9 Copyright 2020 International Barcode of Life Consortium. All Rights Reserved.<\/p>\n

[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

A tool for specimen identification and species discovery The ProcessStep 1:\u00a0Isolate DNA from the sample Step 2:\u00a0Amplify the target DNA barcode region using PCR Step 3: Sequence the PCR products Step 4:\u00a0Compare the resulting sequences against reference databases to find the matching speciesThe DNA Barcode  Plant barcoding studies use one or a few plastid regions […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":13,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

\"\"<\/strong><\/p>\r\n

Step 1:\u00a0<\/em>Isolate DNA from the sample<\/strong>\r\nStep 2:<\/em>\u00a0Amplify the genomic region of interest (the DNA barcode) using universal primer pairs<\/strong>\r\nStep 3:<\/em> Sequence the PCR products<\/strong>\r\nStep 4:<\/em>\u00a0Compare the resulting sequences against reference databases to find the matching species<\/strong><\/p>\r\n\u00a0\r\n\r\n\"\"\r\n\r\nThe DNA barcoding being used for almost all animal groups, a region in the mitochondrial cytochrome c<\/em> oxidase 1 gene (\u201cCO1\u201d), is short enough to be sequenced quickly and cheaply yet long enough to identify variations among species.\r\n\r\n\u00a0\r\n\r\n\u00a0\r\n\r\n\"\"\r\n\r\nPlant-barcoding studies use one or a few plastid regions (e.g. the protein coding \u2018core barcodes' rbcL and matK, and the non-coding spacer trnH-psbA) and the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (ITS\u2014either its entirety or just the ITS2 region).\r\n\r\n\u00a0\r\n\r\n\u00a0\r\n\r\n\"\"\r\n\r\nThe formal acceptance of the internal transcribed spacer (ITS) region in the nuclear ribosomal cistron as the standard fungal barcode was based on a phylogenetically wide-ranging test showing reasonable discriminatory power at the species level in many groups.\r\n\r\n\u00a0\r\n\r\nOnce the barcode sequence has been obtained, it is placed in the\u00a0Barcode of Life Data Systems<\/a> (BOLD)\u00a0database \u2013 an online workbench that includes a reference library of DNA barcodes that can be used to assign identities to unknown specimens. BOLD is a searchable repository for barcode records, storing specimen data and images as well as sequences and trace files. It provides an identification engine based on the current barcode library and monitors the number of barcode sequence records and species coverage","_et_gb_content_width":"","footnotes":""},"class_list":["post-17","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/pages\/17","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/comments?post=17"}],"version-history":[{"count":1,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/pages\/17\/revisions"}],"predecessor-version":[{"id":11216,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/pages\/17\/revisions\/11216"}],"up":[{"embeddable":true,"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/pages\/13"}],"wp:attachment":[{"href":"https:\/\/ibol.org\/site\/wp-json\/wp\/v2\/media?parent=17"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}