{"id":3916,"date":"2020-05-06T09:00:50","date_gmt":"2020-05-06T13:00:50","guid":{"rendered":"https:\/\/ibol.org\/barcodebulletin\/?p=3916"},"modified":"2020-05-06T10:24:33","modified_gmt":"2020-05-06T14:24:33","slug":"judge-a-caterpillar-by-what-they-eat-not-where-theyre-found","status":"publish","type":"post","link":"https:\/\/ibol.org\/barcodebulletin\/research\/judge-a-caterpillar-by-what-they-eat-not-where-theyre-found\/","title":{"rendered":"Judge a caterpillar by what they eat, not where they\u2019re found"},"content":{"rendered":"

[et_pb_section fb_built=”1″ fullwidth=”on” _builder_version=”3.27.4″ background_blend=”multiply” custom_padding=”0px|0px|3px|0px|false|false”][et_pb_fullwidth_header title=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF90aXRsZSIsInNldHRpbmdzIjp7ImJlZm9yZSI6IiIsImFmdGVyIjoiIn19@” subhead=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9leGNlcnB0Iiwic2V0dGluZ3MiOnsiYmVmb3JlIjoiIiwiYWZ0ZXIiOiIiLCJ3b3JkcyI6IiIsInJlYWRfbW9yZV9sYWJlbCI6IiJ9fQ==@” _builder_version=”4.4.3″ _dynamic_attributes=”title,subhead” title_font=”|||||on|||” title_line_height=”1.2em” content_font=”||||||||” content_text_color=”rgba(0,0,0,0.8)” subhead_font=”||on||||||” subhead_text_color=”#ffffff” subhead_font_size=”16px” subhead_line_height=”1.5em” background_color=”rgba(168,211,103,0)” use_background_color_gradient=”on” background_color_gradient_start=”#0a1404″ background_color_gradient_end=”#003254″ background_color_gradient_end_position=”70%” background_image=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2019\/04\/v4.boldsystems.org_7125_animalia.png” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop”][\/et_pb_fullwidth_header][et_pb_fullwidth_image src=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2020\/05\/Hausmann-MN.jpg” _builder_version=”4.4.3″ max_width=”95%” module_alignment=”center” custom_margin=”-20px||20px” box_shadow_style=”preset1″][\/et_pb_fullwidth_image][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22.3″ custom_padding=”0|0px|7px|0px|false|false”][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ width=”89%” width_tablet=”80%” width_phone=”” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_phone=”” max_width_last_edited=”on|desktop” custom_padding=”0|0px|27px|0px|false|false” make_fullwidth=”on”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.4.3″ text_font=”||||||||” text_text_color=”#646564″]<\/p>\n

The original primary rainforest surrounding\u00a0Panguana station, Peru, Dept Hu\u00e1nuco, western Amazonia.<\/span><\/p>\n

PHOTO CREDIT: K. Wothe<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ use_custom_gutter=”on” gutter_width=”2″ specialty=”on” _builder_version=”3.27.4″][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_row_inner _builder_version=”3.27.4″][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”3.27.4″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.4.3″ text_font=”||||||||”]<\/p>\n

U<\/span>nderstanding species and their associations with each other and with the environment \u2013 a key aspect of synecological research \u2013 is of great importance. For example, data on insect-host plant relationships can aid investigations into food webs and extrapolations of global species numbers as well as inform forestry, agriculture, and conservation practices.<\/p>\n

Supported by the Bavarian Ministry of Science (\u2018SNSB-Innovativ\u2019), our recent pilot study<\/a> examined the gut contents of Peruvian caterpillars demonstrating the potential for gathering large-scale data on species interactions when applying DNA barcoding and high-throughput sequencing technologies. We obtained 130 caterpillars (moth larvae) by canopy fogging at the Panguana research station<\/a><\/span>, an area of tropical primary forest in western Amazonia. DNA barcode analysis resulted in 119 successfully sequenced larvae, more than half of which matched with moth reference sequences on BOLD<\/a><\/span>. Surprisingly high biodiversity was uncovered from our modest sample \u2013 92 BINs or species proxies. The trees from which caterpillars were collected were also identified, both by morphology and DNA barcoding.<\/p>\n

[\/et_pb_text][et_pb_image src=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2020\/05\/Hausmann-Figure1.jpg” show_in_lightbox=”on” align=”center” _builder_version=”4.4.3″][\/et_pb_image][et_pb_text _builder_version=”4.4.3″ text_font=”||||||||” text_text_color=”#646564″ text_font_size=”16px” custom_padding=”||3px|||”]<\/p>\n

Panguana research station, Peru, Dept Hu\u00e1nuco, western Amazonia, with the characteristic Lupuna tree (Ceiba pentandra<\/em>, Malvaceae) in the background.<\/p>\n

PHOTO CREDIT: J. Diller<\/span><\/p>\n

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Knowing the tree and larva identity is not enough to conclude a host-plant relationship, particularly in a dense tropical rainforest. Caterpillars may in fact be feeding on the epiphytes, lianas, lichens, algae, fungi, or mosses associated with trees (i.e., alternative feeding<\/em>), and sometimes larvae may have been fogged down from neighbouring trees. To confirm a direct insect-host plant relationship, we partnered with the company\u00a0Advanced Identification Methods<\/a>\u00a0(AIM) to design a high-throughput sequencing (HTS) protocol with plant markers (rbcL<\/em>, psbA<\/em>) that would enable the identification of plant matter from the gut contents of ten larvae. Results revealed only two matches between the fogged tree and larval gut content which suggests a rather high percentage of alternative feeding. In three cases, the gut content clearly indicated feeding on lianas and neighbouring trees. Interestingly, the analysis of four larvae resulted in the putative presence of Bryophyta, suggesting moss-feeding in Lepidoptera, a phenomenon rarely observed. Potential contamination (for example, through the diffusion of plant DNA into the alcohol of the bulk sample) has yet to be ruled out, work which is currently being validated in a subsequent project investigating the gut contents of an additional 190 larvae.<\/p>\n

[\/et_pb_text][baie_before_after_image src_before=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2020\/05\/Hausmann-Figure2A.jpg” label_before=” ” src_after=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2020\/05\/Hausmann-Figure2B.jpg” label_after=” ” size=”fullsize.” slider_offset=”52%” align=”center” _builder_version=”4.4.3″ background_enable_color=”off” module_alignment=”center”][\/baie_before_after_image][et_pb_text _builder_version=”4.4.3″ text_font=”||||||||” text_text_color=”#646564″ text_font_size=”16px”]<\/p>\n

Automeris denticulata<\/em> (Conte, 1906) (Saturniidae): Larva (left), selected from canopy fogging bulk samples of a Poulsenia<\/em> (Moraceae) tree at the Panguana station, identified by its COI barcode; Adult (right), collected at the Panguana station.<\/p>\n

PHOTO CREDIT: Mei-Yu Chen &\u00a0Dr. R. M\u00f6rtter<\/span><\/p>\n

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Our approach of combined canopy fogging, DNA-based identification, and gut content analysis resulted in two key findings. First, a significant portion of both insect and plant taxa can be identified even in highly diverse, tropical regions \u2013 more than 97% to a family level and about 80% to a species or genus level. Secondly, we can successfully confirm or reject the hypothesis that caterpillars feed on the trees where they are collected by identifying their diets through an HTS protocol on gut contents. Importantly, the taxonomic resolution of animal and plant identifications will increase with further investments into DNA reference libraries. We recommend specimen de-contamination (e.g. by bleaching) and\/or isolated storage of the target taxa rather than bulk storage to improve the reliability of gut content analysis.<\/p>\n

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Urania leilus<\/em> (Linnaeus, 1758) (Uraniidae): Larva (left), selected from canopy fogging bulk samples of an Oxandra polyantha<\/em> (Annonaceae) tree at the Panguana station, identified by its COI barcode; Adult (right).<\/p>\n

PHOTO CREDIT: Mei-Yu Chen &\u00a0Dr. J. Diller<\/span><\/p>\n

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The techniques employed in our pilot have immense potential for unveiling trophic interactions in tropical regions at a very large scale as they are fast and cost-effective. The latter is enabled, in part, by the availability of target specimens in the by-catch of other studies. For example, our efforts fogging 150 trees in a separate project assessing the biodiversity of ants have resulted in 1,200 lepidopteran larvae. Subsequent aspects of the workflow, from selecting the larvae from bulk samples, tissue sampling, photography, and databasing, required 10\u201320 minutes per larva and can be performed with relatively low expertise. The costs for subsequent lab work, i.e. identification of larvae and their gut contents, currently amount to 20\u201325 \u20ac per larva and these costs will soon drop considerably. In contrast, traditional approaches involving the searching and rearing of larvae, and the identification of hatched adults by experts is massively time and resource consuming.<\/p>\n

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Providing reliable data on trophic interactions is one of the major goals of the BIOSCAN<\/a><\/span> program, one that will be a powerful tool for investigating food webs, for determining the amplitude of alternative or multiple feeding sources, and for the study of phagism (monophagy versus polyphagy), thus gaining data for extrapolations of global species numbers. These data will also be particularly important for pest management in forestry, and agriculture, and for conservation purposes.<\/p>\n

Overcoming the current lack of knowledge is a major challenge, particularly in ecoregions with megadiverse faunas and floras. Yet, its success is imperative for humanity considering the unprecedented biodiversity losses we currently face. In this context, the recently launched BIOSCAN with its focus on revealing species interactions will embolden an important plan for the international research community to come together in understanding nature and conserving it for a sustainable future.<\/p>\n

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Read the complete manuscript in\u00a0PLoS ONE<\/em><\/a>.<\/p>\n

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Written by<\/p>\n

[\/et_pb_text][et_pb_team_member name=”Axel Hausmann” image_url=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2020\/05\/Axel-Hausmann.jpg.png” _builder_version=”4.4.3″ header_font=”||||||||” header_text_align=”center” header_font_size=”17px” body_font=”||||||||” body_font_size=”15px” text_orientation=”center” width=”85%” module_alignment=”center” link_option_url=” https:\/\/www.researchgate.net\/profile\/Lasse_Topstad” link_option_url_new_window=”on”][\/et_pb_team_member][et_pb_team_member name=”Juliane Diller ” image_url=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2020\/05\/Juliane-Diller.png” _builder_version=”4.4.3″ header_font=”||||||||” header_text_align=”center” header_font_size=”17px” body_font=”||||||||” body_font_size=”15px” text_orientation=”center” width=”85%” module_alignment=”center” link_option_url=” https:\/\/www.researchgate.net\/profile\/Lasse_Topstad” link_option_url_new_window=”on”][\/et_pb_team_member][et_pb_team_member name=”Amelie H\u00f6cherl” position=”SNSB \u2013 Staatliche Naturwissenschaftliche Sammlungen Bayerns – Zoologische Staatssammlung M\u00fcnchen, Munich, Germany” image_url=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2020\/05\/Amelie-H\u00f6cherl.png” _builder_version=”4.4.3″ header_font=”||||||||” header_text_align=”center” header_font_size=”17px” body_font=”||||||||” body_text_color=”#969693″ body_font_size=”15px” text_orientation=”center” width=”85%” module_alignment=”center” link_option_url=” https:\/\/www.researchgate.net\/profile\/Lasse_Topstad” link_option_url_new_window=”on”][\/et_pb_team_member][et_pb_divider divider_position=”center” _builder_version=”3.23.3″ custom_margin=”||1px”][\/et_pb_divider][et_pb_text _builder_version=”3.27.4″ _dynamic_attributes=”content” text_font=”||||||||” text_text_color=”#003254″ text_orientation=”center”]@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9kYXRlIiwic2V0dGluZ3MiOnsiYmVmb3JlIjoiIiwiYWZ0ZXIiOiIiLCJkYXRlX2Zvcm1hdCI6ImRlZmF1bHQiLCJjdXN0b21fZGF0ZV9mb3JtYXQiOiIifX0=@[\/et_pb_text][et_pb_button button_url=”https:\/\/ibol.org\/barcodebulletin\/wp-content\/uploads\/2020\/05\/2020-Hausmann.pdf” url_new_window=”on” button_text=”Download PDF” button_alignment=”center” disabled_on=”off|off|off” _builder_version=”4.4.3″ custom_button=”on” button_text_size=”12px” button_text_color=”#ffffff” button_bg_color=”#003254″ button_icon=”%%26%%” button_icon_color=”#ffffff” box_shadow_style=”preset3″ box_shadow_horizontal=”3px” box_shadow_vertical=”11px”][\/et_pb_button][et_pb_text _builder_version=”4.4.3″ text_font=”||||||||” text_text_color=”#646564″ text_font_size=”12px” text_orientation=”center”]<\/p>\n

https:\/\/doi.org\/10.21083\/ibol.v10i1.6133\u00a0<\/span><\/pre>\n
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Gut content analysis of Peruvian caterpillars reveals new insights into host-plant relationships and the methods used to examine species interactions key to BIOSCAN<\/p>\n","protected":false},"author":19,"featured_media":3918,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n[twenty20 img1=\u201d3950\u2033 img2=\u201d3951\u2033 direction=\u201dhorizontal\u201d offset=\u201d0.4\u2033 align=\u201dnone\u201d width=\u201d100%\u201d before=\u201dBefore\u201d after=\u201dAfter\u201d hover=\u201dfalse\u201d]\n","_et_gb_content_width":"2880","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[2],"tags":[307,311,315,313,316,306,277,312],"coauthors":[161,304,305],"class_list":["post-3916","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research","tag-amazonia","tag-diet-analysis","tag-high-throughput-sequencing","tag-larva","tag-lepidoptera","tag-peru","tag-species-interactions","tag-trees"],"_links":{"self":[{"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/posts\/3916","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/comments?post=3916"}],"version-history":[{"count":37,"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/posts\/3916\/revisions"}],"predecessor-version":[{"id":4005,"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/posts\/3916\/revisions\/4005"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/media\/3918"}],"wp:attachment":[{"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/media?parent=3916"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/categories?post=3916"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/tags?post=3916"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/ibol.org\/barcodebulletin\/wp-json\/wp\/v2\/coauthors?post=3916"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}