Terminal restriction fragment length polymorphism (T-RFLP) uses PCR amplification of DNA using primer pairs that have been labelled with fluorescent tags. The amplified PCR products are then digested using restriction enzymes. The resulting patterns are then visualized using a DNA sequencer and analyzed either by comparing bands or peaks from the T-RFLP runs to a database of known species. This method has been applied for characterizing bacterial communities in mixed-species samples.